Laboratorio de Enzimología

 

Inicio     Curso Bioquímica II     Curso Enzimología     Investigación

 

Publicaciones  

 

Dra. Beatriz Alvarez 

Carballal, S., Trujillo, M., Cuevasanta, E., Bartesaghi, S., Möller, M. N., Folkes, L., García-Bereguiaín, M. A.., Gutiérrez-Merino, C., Wardman, P., Denicola, A., Radi, R. and Alvarez, B. 2010. Reactivity of hydrogen sulfide with peroxynitrite and other oxidants of biological interest. Free Radical Biology and Medicine, en revisión.

Celano, L., Gil, M., Carballal, S., Durán, R., Denicola, A., Banerjee, R. and Alvarez, B. Inactivation of cystathionine β-synthase with peroxynitrite. 2009. Archives of Biochemistry and Biophysics 491(1-2):96-105.  

Hugo, M., Turell, L., Manta, B., Botti, H., Monteiro, G., Netto, L. E. S., Alvarez, B., Radi, R. and Trujillo, M. Thiol and sulfenic acid oxidation of AhpE, the one-cysteine peroxiredoxin from Mycobacterium tuberculosis: kinetics, acidity constants and conformational dynamics. 2009. Biochemistry 48(40):9416-26.

González, S., Fló, M., Margenat, M., Durán, R., Graña, M:, González, G., Parkinson, J., Maizels, R., Salinas, G., Alvarez, B. and Fernández, C. A family of diverse Kunitz inhibitors from Echinococcus granulosus involved in host-parasite cross-talk in echinococcosis. 2009. PLoS ONE  4(9):e7009.

Alvarez, B., Carballal, S., Turell, L. and Radi, R. 2010. Formation and reactions of sulfenic acid in human serum albumin. Methods in Enzymology 473, 117-136.

Trujillo, M., Alvarez, B., Souza, J. M-, Romero, N., Castro, L., Thomson, L. and Radi, R. 2010. Mechanisms and biological consequences of peroxynitrite-dependent protein oxidation and nitration. En Ignarro, L. J. (Ed.): Nitric Oxide: Biology and Pathobiology II, Academic Press, 61-102.

Turell, L., Botti, H., Carballal, S., Radi, R. and Alvarez, B. 2009. Sulfenic acid - A key intermediate in albumin thiol oxidation. J Chromatogr B Analyt Technol Biomed Life Sci. 877(28):3384-92.

Turell, L., Carballal, S., Botti, H., Radi, R. and Alvarez, B. 2009. Oxidation of the albumin thiol to sulfenic acid and its implications in the intravascular compartment. Brazilian Journal of Medical and Biological Research, 42: 305-311.

Denicola, A., Alvarez, B. and Thomson, L. 2008. 3-Nitrotyrosine, a post-translational modification associated with nitroxidative stress. En Free Radical Pathophysiology (S. Alvarez, P. Evelson and Boveris, A, eds.) Transworld Research Network, pp. 39-55.

Carballal, S., Madzelan, P., Zinola, C.F., Graña, M., Radi, R., Banerjee, R. and Alvarez, B. 2008. Dioxygen reactivity and heme redox potential of truncated human cystathionine beta-synthase. Biochemistry 47(10):3194-3201.

Turell, L., Botti, H., Carballal, S., Ferrer-Sueta, G., Souza, J. M., Durán, R., Freeman, B. A., Radi, R. and Alvarez, B. 2008. Reactivity of sulfenic acid in human serum albumin. Biochemistry 47(1):358-67.

Denicola, A., Alvarez, B. and Thomson, L. 2008. 3-Nitrotyrosine, a post-translational modification associated with nitroxidative stress. En Free Radical Pathophysiology (S. Alvarez, P. Evelson, eds.) Transworld Research Network, en prensa.

Demicheli, V., Quijano, C., Alvarez, B. and Radi, R. 2007. Inactivation and nitration of human superoxide dismutase (SOD) by fluxes of nitric oxide and superoxide. Free Radical Biology and Medicine, 42: 1359-68.

Carballal, S., Alvarez, B., Turell, L., Botti, H., Freeman, B. A. and Radi, R. 2007. Sulfenic acid in human serum albumin. Amino Acids 32: 543-51.

Alvarez, B., Demicheli, V., Duran, R., Trujillo, M., Cervenansky, C., Freeman, B. A., Radi,  R. 2004. Inactivation of human Cu,Zn superoxide dismutase by peroxynitrite and formation of histidinyl radical. Free Radical Biology and Medicine, 37(6): 813-22.

Lang, J. D. Jr., Figueroa, M., Chumley, P., Aslan, M., Hurt, J., Tarpey, M. M., Alvarez, B., Radi R. and Freeman, B. A. 2004. Albumin and hydroxyethyl starch modulate oxidative inflammatory injury to vascular endothelium. Anesthesiology,  100(1): 51-8.

Carballal, S., Radi, R., Kirk, M. C., Barnes, S., Freeman, B. A. and Alvarez, B. 2003. Sulfenic acid formation in human serum albumin by hydrogen peroxide and peroxynitrite. Biochemistry, 42:9906-9914.

Alvarez, B. and Radi, R. 2003. Peroxynitrite reactivity with amino acids and proteins. Amino Acids   25(3-4): 295-311.

Naya, H., Romero, H., Zavala, A., Alvarez, B. and Musto, H. 2002. Aerobiosis increases genomic GC % in prokaryotes. Journal of  Molecular Evolution,  55: 260-264.

Ferrer-Sueta, G., Quijano, C., Alvarez, B. and Radi, R. 2002. Reactions of manganese porphyrins and Mn superoxide dismutase with peroxynitrite. Methods in Enzymology 349: 23-37.

Blanchard-Fillion, B., Souza, J. M., Friel, T., Jiang, G. C, Vrana, K., Sharov, V., Baron, L.,Schoneich, C., Quijano, C., Alvarez, B., Radi, R., Przedborski, S.,Fernado, G. S., Horwitz, J. and Ischiropoulos, H.  2001. Nitration and inactivation of tyrosine hydroxylase by peroxynitrite. Journal of Biological Chemistry 276(49): 46017-23.

Alvarez, B. and Radi, R. 2001. Peroxynitrite decay in the presence of hydrogen peroxide, mannitol and ethanol: a reappraisal. Free Radical Research, 34(5): 467-75.

Radi, R., Denicola, A., Alvarez, B., Ferrer-Sueta, G. and Rubbo, H. 2000. The Biological Chemistry of Peroxynitrite. En Ignarro, L. J. (Ed.): Nitric Oxide: Biology and Pathobiology, Academic Press, pp. 57-82.

Schöpfer, F., Riobó, N., Carreras, M. C., Alvarez, B., Radi, R., Boveris, A., Cadenas, E. and Poderoso, J. J. 2000. Oxidation of ubiquinol by peroxynitrite: implications for protection of mitochondria against  nitrosative damage. Biochemical Journal,  349(1): 35-42.

Alvarez, B., Ferrer-Sueta, G., Freeman, B. A. and Radi, R. 1999. Kinetics of peroxynitrite reaction with amino acids and human serum albumin as a model protein. Journal of Biological Chemistry, 274(2): 842-848.

Alvarez, B., Ferrer-Sueta, G. and Radi, R. 1998. Slowing of peroxynitrite decomposition in the presence of mannitol and ethanol. Free Radical Biology and Medicine, 24 (7-8): 1331-1337.

Gatti, R. M., Alvarez, B., Vasquez-Vivar, J., Radi, R. and Augusto, O. 1998. Formation of spin trap adducts during the decomposition of peroxynitrite. Archives of Biochemistry and Biophysics, 349: 36-46.

Quijano, C., Alvarez, B., Gatti, R. M., Augusto, O. and Radi, R. 1997. Pathways of  peroxynitrite oxidation of sulfhydrils. Biochemical Journal. 322: 167-173. 

Alvarez, B., Rubbo, H., Kirk, M., Barnes, S., Freeman, B. A. and Radi, R. 1996. Peroxynitrite-dependent tryptophan nitration. Chemical Research in Toxicology,  9(2): 390-396.

Alvarez, B., Denicola, A. and Radi, R. 1995. Reaction between peroxynitrite and hydrogen peroxide: formation of oxygen and slowing of peroxynitrite decomposition. Chemical Research in Toxicology, 8(6): 859-869.

Ureta, A., Alvarez, B., Ramón, A., Vera, M. A. and Martínez-Drets, G. 1995. Identification of Acetobacter diazotrophicus, Herbaspirillum seropedicae and Herbaspirillum rubrisubalbicans using biochemical and genetic criteria. Plant and Soil, 172: 271-277.

Alvarez, B. and Martínez-Drets, G. 1995. Metabolic characterization of Acetobacter diazotrophicus. Canadian Journal of Microbiology, 41(10): 918-924.